Proceedings of the 41st International Academic Conference, Venice

IN VIVO GASTROPROTECTIVE STUDY OF SELECTED ANTIOXIDANTS, VITAMINS AND MINERALS USING PYLORUS LIGATION INDUCED ULCERETED RATS.

DARSHAN SHAH

Abstract:

This investigation aimed to study the effect of selected antioxidants, vitamins and minerals on gastroprotection using pylorus ligation induced gastric mucosal damage in rats. The anti ulcerogenic effect of test substances was investigated in Albino Wistar rats, weighing between 200-250 g. were divided into 9 groups of 6 animals each (n=6). The groups were treated respectively as follows Group I normal control and Group II Positive control received normal saline, Group III was treated with standard drug Omeprazole, Group IV to IX received test substances respectively, two antioxidants (Vitamin E and Cystine) two vitamins (Vitamin B1 and Niacinamide) & two minerals (Iron and Zinc) for 5 days. Ulceration was induced by pylorus ligation. Various parameters like, the volume, pH of gastric juice, total acidity, ulcer index, percentage protection, biochemical parameters like mucin content, pepsin activity and antioxidant enzymes like Super oxide dismutase, catalase, reduced glutathione, myeloperoxidase, and malondialdehyde were estimated. Histopathology of stomach epithelium was observed. The ulcer index and total acidity were significantly reduced (p<0.05) increase in pH was observed in ulcer induced rats pretreated with test substances. Mucin content was found to be restored significantly in all rats pretreated with test substances, and pepsin activity was decreased significantly (p<0.05) when compared with positive control rats. The alteration observed in the level of super oxide dismutase, catalase, reduced glutathione were increased in test substances treated rats and showed significant restoration. The level of myeloperoxidase and malondialdehyde were decreased significantly (p<0.05). Histopathology of gastric mucosa confirmed the gastroprotection by test substances. The study reveals antiulcerogenic effects were observed in all test groups antioxidants (Vitamin E and L- Cystine), vitamins (vitamin B1 and Niacinamide) and minerals (Iron and Zinc) probably by its free radical scavenging activity, antisecretory activity, cytoprotection and mucin preventing nature.

Keywords: Antioxidants, Histopathology, Indomethacin, Myeloperoxidase, Minerals, Vitamins.

DOI: 10.20472/IAC.2018.041.030

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